Primer Designer allows users to design new target-specific primers in one step as well as to check the specificity of pre-existing primers with BLAST.
This tool was designed to test the specificity of any primer pair on the coffee genome by using blast. Make sure primers have the same id in both cases. As a result, a table displays all tested primers with primer name and positions, location on chromosome, amplicon size and number of hits on the musa genome. If the primer pair is really specific, this status is clearly marked as ‘ok’ in the table.
Search for highest similarity for your sequence of interest.
Allows users to navigate genome sequence, annotation, and associated data track sequence, annotation, and associated data tracks .
Search for a gene by name, location, functional annotation keywords...